Novi Primadewi, Harijono KS, Ari Natalia Probandari, Budiyanti W
Cisplatin is an anti-cancer which has good results for the treatment of malignancy cases. But the accumulation of excessive reactive oxygen species (ROS) will result in the inefficiency and impaired function of the antioxidant enzymes synthesis. The aim of this study is to determine expression of the decreased levels of the antioxidant enzyme, Superoxide Dismutase (SOD) Catalase (CAT), glutathione peroxidase (GPx) and damage to lipid peroxidase as indicated by increased levels of Malondialdehyde (MDA). When the antioxidant enzyme system unable to cope with excessive oxidative stress, the body requires intake of exogenous antioxidants that are able to capture and neutralize these free radicals so that further reactions that cause oxidative stress can stop and cell damage can be avoided. Ginkgo biloba, as well as combinations Camellia sinensis L in green tea and Curcuma longa in turmeric. All three of them are polyphenols, rich in exogenous antioxidant content, so they are expected to reduce oxidative stress due to cisplatin induction. This was a randomized post-test only control group laboratory experimental design in rat to determine the effectiveness of intravenous Ginkgo biloba, as well as combinations Curcuma longa L and Camellia sinensis administration can reduce oxidative stress based on the activity of the antioxidant enzyme Superoxide Dismutase (SOD) Catalase (CAT), Glutathione Peroxidase (GPx) and Malondialdehyde (MDA) in blood serum levels. 25 male Rattus norvegicus Wistar strain divided randomly into 5 groups, each group consisting of 5 rats, group 1 as a control group without treatment, group 2 was only given cisplatin 20 mg/kgBW/iv, group 3 was given cisplatin 20 mg/kgBW/iv continued with combination therapy Cammelia Senensis Extract at a dose of 50 mg/KgBW/day and Curcuma Longa L Extract at a dose of 75 mg/KgBW/day; Group 4 was given cisplatin 20 mg/kgBW/iv followed by combination therapy Cammelia Sinensis Extract at a dose of 75 mg/KgBW/day and Curcuma Longa Extract at a dose of 100 mg/KgBW/day, group 5 was given cisplatin 20 mg/kgBW/iv followed by therapy with Ginkgo Biloba at a dose of 1.44 mg/KgBW/day. All groups were terminated on day 7. Data were analyzed statistically using SPSS. Data were analyzed using ANOVA and p <0.05 was used. Combination Curcuma longa L in turmeric and Camellia sinensis L in green tea showed its effectiveness in reducing oxidative stress on the 7th day after administration, as evidenced by the highest levels of Superoxide Dismutase (SOD) Catalase (CAT), glutathione peroxidase (GPx) expression and the lowest levels of MDA expression.